Hydrogels containing porphyrin-loaded nanoparticles for topical photodynamic applications.

摘要: Abstract 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)-porphyrin tetra-iodide (TMPyP), a potent water-soluble photosensitizer (PS) used in antimicrobial applications, was encapsulated into poly(lactic-co-glycolic acid) (PLGA) nanoparticles (TMPyP-PLGA) for topical delivery purposes. Nanoparticles resulted mean particle size around 130 nm, narrow polydispersity index (PdI), spherical morphology and association efficiency up to 93%. Free TMPyP TMPyP-PLGA were incorporated Carbopol® hydrogels, resulting controlled release of about 60% 20% after 4.5 h, respectively. Critical properties such as appearance, clarity, viscosity pH maintained over time, hydrogels stable during 6 months at 4 °C, 25 °C/60% RH 40 °C/75% RH. For photodynamic the photoproduction singlet oxygen from these quite efficient being both formulations very photostable 20 min. No permeation through pig ear skin observed 24 h, histological assays did not show relevant damages surrounding tissues. All excellent characteristics make them promising platforms applications clinical use.