Cellulose-degrading bacteria associated with the invasive woodwasp Sirex noctilio

摘要: Sirex noctilio is an invasive wood-feeding wasp that threatens the world's commercial and natural pine forests. Successful tree colonization by this insect contingent on decline of host defenses ability to utilize woody substrate as a source energy. We explored its potential association with bacterial symbionts may assist in nutrient acquisition via plant biomass deconstruction using growth assays, culture-dependent -independent analysis frequency whole-genome analysis. identified Streptomyces γ-Proteobacteria were each associated 94% 88% wasps, respectively. isolates grew all three cellulose substrates tested across range pH 5.6 9. On basis sequencing, have some highest proportions genes predicted encode for carbohydrate-active enzymes (CAZyme) sequenced Actinobacteria. derivative structurally diverse substrate, ammonia fiber explosion-treated corn stover, but not microcrystalline cellulose. Analysis genome Pantoea isolate detected putatively encoding CAZymes, majority be active hemicellulose more simple sugars. propose consortium microorganisms, including described bacteria fungal symbiont Amylostereum areolatum, has complementary functions degrading such degradation S. noctilio, thus contributing established forested habitats worldwide.