Surface, anchored fc-bait antibody display system

作者: Dongxing Zha , Hussam Hisham Shaheen

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摘要: The present invention provides, in part, an antibody display system that simultaneously uses a secretion and mode. A bait complexed with monovalent fragment can be expressed on the surface of host cell wherein may assayed for antigen binding while full is secreted from cell. Methods using identifying antibodies bind specifically to interest are also provided. Polypeptides, polynucleotides cells useful making provided along methods use thereof.

参考文章(24)
Michael Krause, Transcription and translation. Methods in Cell Biology. ,vol. 48, pp. 483- 512 ,(1995) , 10.1016/S0091-679X(08)61400-4
David M. Glover, DNA cloning : a practical approach IRL Press. ,(1985)
Harald Kolmar, Thorsten Adams, Means and methods for displaying polypeptides on cells ,(2006)
Hideyuki Ohi, Taeko Miwa, Kenmi Miyano, Masami Miura, Masaaki Hirose, Hiroyuki Irie, Wataru Otani, Shinobu Kuwae, Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby ,(2001)
Piotr Bobrowicz, W. James Cook, Warren Kett, Production of glycoproteins with reduced o-glycosylation ,(2006)
Piotr Bobrowicz, Stefan Wildt, Dongxing Zha, Natarajan Sethuraman, Bianka Prinz, Surface display of whole antibodies in eukaryotes ,(2009)
Peter Peizhi Luo, Pingyu Zhong, Kevin Caili Wang, Jian Wang, Eukaryotic cell display systems ,(2008)
P. Davanloo, A. H. Rosenberg, J. J. Dunn, F. W. Studier, Cloning and expression of the gene for bacteriophage T7 RNA polymerase Proceedings of the National Academy of Sciences of the United States of America. ,vol. 81, pp. 2035- 2039 ,(1986) , 10.1073/PNAS.81.7.2035