Impact of New Microbial PR/PGP Inducers on Increase of Resistance to Parasitic Nematode of Wild and RNAi Transgenic Rape Plants

作者: V.A. Tsygankova , O.N. Bondarenko , Ya.B. Blume , O.A. Babich , A.P. Galkin

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摘要: The bioprotective effect against parasitic nematode Heterodera schachii Schmidt of new microbial PR/PGP inducers on the wild spring rape plants Kalinivsky cultivar and RNAi transgenic was studied. In field conditions artificially created invasive background considerable diminishing amount nematodes roots up to 89.7 % - under action Avercom 38.5 Avercom-nova 2 found. increasing productivity grain yields infected by 2775.6 pounds/ha 2156.1 compared control observed. Using Dot-blot hybridization method increase difference in degree homology between cytoplasmic mRNA from small regulatory si/miRNA treated with Avercom, nova-1 nova-2 plants: 30-39 for 38-47 wheat embryo cell-free system protein synthesis silencing activity isolated these 26-43 58-86 templates both plant revealed. Results study indicate that specific anti-nematodic plants. Owing this process resistance RNAi-transgenic occurs.

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