From mouse to moose: Multilocus genotyping of Giardia isolates from various animal species

摘要: Giardia intestinalis is a protozoan parasite that consists of seven genetically distinct assemblages (A to G). Assemblage A and B parasites have been detected in wide range animals including humans, while the other (C G) appear narrower host range. However, knowledge about zoonotic transmission G. limited. To address this question, 114 isolates from various Sweden pets, livestock, wildlife captive non-human primates were investigated by sequence-based analysis three genes (beta-giardin, glutamate dehydrogenase triose phosphate isomerase). infections nine ruminants, five cats one dog, sheep infected with both E. Multilocus genotypes (MLGs) defined for assemblage A, these MLGs previously humans. The newly described sub-assemblage AIII, until now reported mainly wild hoofed animals, was found cat isolate. occurred monkeys, guinea pig rabbit. rabbit isolate exhibited sequences at all loci human isolates. non-zoonotic C, D, E, F or G remaining 83 isolates, which successfully amplified genotyped, generating variety novel known sub-genotypes. Double peaks chromatograms seen B, D E but never observed can reflect differences allelic sequence divergence. No evidence genetic exchange between detected. study shows multilocus genotyping highly discriminatory useful tool determination sub-groups within less valuable subtyping due high frequency double chromatograms. obtained data also suggest might occur limited extent Sweden.