RecA-mediated annealing of single-stranded DNA and its relation to the mechanism of homologous recombination.
作者： Berndt Müller , Andrzej Stasiak
DOI: 10.1016/0022-2836(91)80210-L
关键词:
摘要: We demonstrate that RecA protein can mediate annealing of complementary DNA strands in vitro by at least two different mechanisms. The first mechanism predominates under conditions where causes coaggregation single-stranded (ssDNA) molecules and RecA-free ssDNA stretches are present on both reaction partners. Under these take place between locally concentrated protein-free sequences. Other aggregating agents like histone H1 or ethanol stimulate the same mechanism. second RecA-mediated is best manifested when preformed saturated RecA-ssDNA complexes interact with ssDNA. In this case, occur strand resident complex interacts complex. Here, action reflects its specific recombination promoting This enables presynaptic RecA-DNA to be exposed for hydrogen bond formation contacting filament.
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