MicroRNA-125b promotes tumor metastasis through targeting tumor protein 53-induced nuclear protein 1 in patients with non-small-cell lung cancer

摘要: Lung cancer, predominantly non-small-cell lung cancer (NSCLC), is the leading cause of deaths worldwide. There a great need to identify critical effectors involved in metastasis NSCLC that will facilitate development new therapeutic strategies. Here we evaluated potential role miR-125b cells. Human cells were isolated from surgical tissues with Cancer Cell Isolation Kit. Expressions and TP53INP1 detected real-time PCR western blot. mimics, inhibitor, expression plasmid siRNA transfected into nucleofector transfection kit. was determined adhesion assay, invasive assay tumor model. The significantly higher poorly differentiated are endowed high metastatic potentials. Up-regulation could enhance vitro vivo, while down-regulation resulted decreased potentials vivo. Further, protein 53-induced nuclear 1 (TP53INP1) an important target served as negative regulator metastasis. Decreased inversely associated their miR-125b, lower tumors correlated clinical stages patients NSCLC. These findings demonstrated promoted via targeting human cells, which uncovered real relevance microRNAs biology, provided novel candidates for practice.