MicroRNA-125b promotes tumor growth and suppresses apoptosis by targeting DRAM2 in retinoblastoma.

摘要: PurposeIt is known that microRNAs (miRNAs) are a class of small, noncoding RNAs act as key regulators in various physiological and pathological processes. However, the regulatory mechanisms involving miRNAs retinoblastoma (RB) remain largely unknown. The miRNA miR-125b dysregulated human cancers such breast cancer, hepatocellular carcinoma, ovarian colorectal cancer. significance RB has not been sufficiently investigated. Our objective was to explore role RB.MethodsIn this study, we measured levels using real-time polymerase chain reaction cell lines, including HXO-Rb44, Y79, SO-RB50, normal retinal pigment epithelial line ARPE-19; total 38 pairs primary tissues adjacent noncancerous were also measured. In addition, overexpression lines performed determine RB.ResultsWe found significantly upregulated RB, closely associated with tumor proliferation apoptosis. apparently promotes migration vitro. Gain-of-function vitro experiments further showed mimic suppressed A subsequent dual-luciferase reporter assay identified suppressor gene DRAM2 direct target miR-125b.ConclusionsOur data collectively demonstrate can promote by downregulating DRAM2, indicating may represent new potential diagnostic therapeutic for treatment.