Investigating molecular alterations to profile short- and long-term recurrence-free survival in patients with primary glioblastoma

摘要: Glioblastoma (GB) is the most aggressive type of primary brain tumor. Despite progress in recent years regarding diagnosis and treatment GB, recurrence rate remains high, due to infiltrative dispersive nature tumor, which typically results poor patient prognosis. In present study, 19 formalin-fixed, paraffin-embedded GB samples were selected from patients with tumors. The classified into a short or long recurrence-free survival (RFS) group, based on time first disease patients. molecularly characterized for mutations isocitrate dehydrogenase 1 (IDH1) gene, amplification epidermal growth factor receptor (EGFR) presence EGFR variant III, methylation promoter region O6-methylguanine-DNA methyltransferase (MGMT) gene. Then, expression 84 genes involved cell-cell cell-matrix interactions, that microRNAs (miRNAs) associated cancer, was profiled. addition, copy number variation analysis 23 reported undergo frequent genomic alterations human glioma also performed. Differences levels detected across RFS groups. Among these genes, 5 particular selected, 5-genes combination approach developed, able differentiate between outcome. high sensitivity precision displayed by this approach, confirmed cross-validation method, provide strong foundation further validation involvement aforementioned larger population. conclusion, study has demonstrated how pattern miRNAs mRNAs defines molecular hallmark may increase reduce behavior tumors, thus influencing rates their response therapy tendency suffer relapse.