Sequencing of the Hepatitis C Virus: A Systematic Review

摘要: Since the identification of hepatitis C virus (HCV), viral sequencing has been important in understanding HCV classification, epidemiology, evolution, transmission clustering, treatment response and natural history. The length diversity genome resulted analysis certain regions virus, however there little standardisation protocols. This systematic review was undertaken to map location frequency on peer reviewed publications, with aim produce a database primers amplicons inform future research. Medline Scopus databases were searched for English language publications based keyword/MeSH terms related sequence (9 terms) or (3 terms), plus “primer” as general search term. Exclusion criteria included non-HCV research, articles, duplicate records, incomplete description methods. PCR primer locations accepted noted, purpose determined. A total 450 studies from 2099 identified, 629 identified mapped genome. most commonly sequenced region HVR-1 region, often utilised history, clustering/transmission, evolution response. Studies genotyping/classification epidemiology genotype generally targeted 5′UTR, Core NS5B regions, while response/resistance assessed mainly NS3–NS5B emphasis Interferon sensitivity determining (ISDR) NS5A. While is constricted consistency positioning primers, exception few highly referenced manuscripts. study demonstrates heterogeneity sequencing, providing comprehensive previously published sets be studies.