CREB‐binding protein regulates lung cancer growth by targeting MAPK and CPSF4 signaling pathway

摘要: CBP (CREB-binding protein) is a transcriptional co-activator which possesses HAT (histone acetyltransferases) activity and participates in many biological processes, including embryonic development, growth control homeostasis. However, its roles the underlying mechanisms regulation of carcinogenesis tumor development remain largely unknown. Here we investigated molecular potential targets involved survival lung cancer cells. Elevated expression was detected cells tissues compared to normal tissues. Knockdown by siRNA or inhibition using specific chemical inhibitor effectively suppressed cell proliferation, migration colony formation induced apoptosis inhibiting MAPK activating cytochrome C/caspase-dependent signaling pathways. Co-immunoprecipitation immunofluorescence analyses revealed co-localization interaction between CPSF4 (cleavage polyadenylation factor 4) proteins inhibited hTERT transcription CBP, vice versa, demonstrating synergetic effect survival. Moreover, found that high both predicted poor prognosis patients with adenocarcinomas. Collectively, our results indicate regulates targeting