Synergistic effects of polymyxin and ionic liquids on lipid vesicle membrane stability and aggregation.

摘要: Ionic liquids (ILs) have been investigated for potential antibacterial and antibiotic applications due to their ability destabilize permeabilize the lipid bilayers in cell membranes. Bacterial assays shown that combining ILs with antibiotics can provide a synergistic enhancement of activities. We characterized mechanism by which conventional 1-butyl-3-methylimidazolium chloride (BMICl) tetrafluoroborate (BMIBF4) enhance membrane permeabilization well-known polymyxin B (PMB). studied sizes permeabilities multilamellar unilamellar bilayer vesicles presence alone aqueous solution, PMB alone, combined together PMB. Light scattering-based experiments show vesicle dramatically increase when are PMB, suggests materials combine synergistically disruption leading aggregation. Lipid leakage using tris (2,2'-bipyridyl) ruthenium (II) (Ru(bpy)32+) trapped vesicles, Ru(bpy)32+ fluorescence lifetime increases it leaks out vesicle, BMIBF4 significantly more than or IL alone. This demonstrates assist could explain increased activities solution.