Reexamination of the role of autolysis in the development of Myxococcus xanthus.

摘要: It has been widely reported that 80 to 90% of the cell population undergoes autolysis during sporulation in Myxococcus xanthus. A re-evaluation techniques used measure M. xanthus showed methods previously draw this conclusion are subject artifacts, which result a substantial underestimation number cells present development. We found at least 80% enter development survive throughout fruiting body formation. The loss did occur appeared be gradual over period 7 days. Our results suggest is not an obligate stage data also sporulating pass through prespore they become osmotically and physically fragile therefore difficult harvest intact. fragility was correlated with change from rod spherical shape. As prespores differentiated into refractile spores, lost became amenable harvesting by standard protocols.