Apoptosis in interface membranes of aseptically loose total hip arthroplasty.

摘要: The terminal events leading to periprosthetic osteolysis are multifactorial in nature and modulation of this process after the stage osteolytic mediator release has been futile. Recently, demonstration ability bisphosphonates inhibit bone resorption that is mediated by particle-stimulated macrophages their induction osteoclast apoptosis suggests a potent area for at prosthesis-bone interface. purpose study was determine mode cell death occurs interface failed total hip arthroplasty (THA). TUNEL staining, DNA laddering, immunodetection poly(ADP-ribose)polymerase (PARP) protein were used identify presence membranes from 25 patients aged 28-88 years old (mean, 58 years) harvested time revision surgery. Our results demonstrated positive stain 100% specimens with an average 37% cells (range 12-60%) positively stained whereas less than 8% control tissue showed staining. characteristic feature apoptotic cells, observed 82% (28/34) studied both acetabular femoral side aseptically loose THAs. No laddering tissues. Finally, using Western blot analysis, we appearance 89 kDa PARP fragment associated 92% (30/33). demonstrate THAs suggesting apoptosis-related indeed could serve as specific target point therapeutic modulation.