Bisphosphonate Inhibition of bone resorption induced by particulate biomaterial-associated macrophages

摘要: Aseptic loosening of total joint replacements is associated with bone resorption. A heavy infiltrate foreign body macrophages in response to biomaterial wear particles commonly found the fibrous membrane surrounding loose components. It has recently been shown that can differentiate into osteoclastic cells. To determine whether pharmacological inhibitors resorption have a role play controlling osteolysis aseptic loosening, we analyzed effect bisphosphonate, disodium ethane-1 , 1 -diphosphonate (EHDP) on this process. Murine monocytes and (derived from granulomas formed by subcutaneous implantation prosthetic biomaterials) were co-cultured UMR106 osteoblast-like cells presence 1,25 dihydroxyvitamin D, for 14 days glass coverslips slices. EHDP significantly inhibited these co-cultures. There was little or no expression osteoclast-associated enzyme, tartrate-resistant acid phosphatase (TRAP) EHDP-treated Addition monocyte-UMR106 co-cultures after appearance TRAP-positive did not abolish resorption, indicating EHDP, addition its known inhibitory osteoclast function, suppresses differentiation precursors. inhibition induced particulate biomaterial-associated shows might be used control loosening.