In-vitro osteogenesis of synovium stem cells induced by controlled release of bisphosphate additives from microspherical mesoporous silica composite.
作者： Xuetao Shi , Yingjun Wang , Rohan R. Varshney , Li Ren , Feng Zhang
DOI: 10.1016/J.BIOMATERIALS.2009.04.021
关键词:
摘要: Abstract In this study, in-vitro osteogenesis was successfully induced in the highly chondrogenic synovium mesenchymal stem cells (SMSCs) by controlled release of a nitrogenous bisphosphonate additive – alendronate (AL) from mesoporous silica (MS)–hydroxyapatite (HA) composite that mediated poly(lactic-co-glycolic acid) (PLGA) microspheres. This microspherical based system is constructed with three levels degradable structures: (1) AL drug first hybridized HA nanoparticles; (2) HA–AL complexes were filled into mesopores MS particles self-assembly situ; and (3) HA–AL-laden constructs (MSH–AL) built bulk PLGA comparison any mono-component construct, superiority multi-component comes two aspects functionalities: significantly greater loading capacity extremely hydrophilic drug-AL; better profile release. Based on newly developed PLGA/MSH–AL releasing system, as recipients SMSCs, which usually exhibit exclusively high chondrogenesis, demonstrated strong osteogenic commitment. The results verified alkaline phosphatase (ALP) activity assay, calcium secretion real time PCR immunohistochemistry analysis. Considering renewable source proliferative achievement engineered SMSC would open new door to major bony reparation regeneration.
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