Functional consequences following infection of the olfactory system by intranasal infusion of the olfactory bulb line variant (OBLV) of mouse hepatitis strain JHM.

摘要: The present study assessed the functional consequences of viral infection with a neurotropic coronavirus, designated MHV OBLV, that specifically targets central olfactory structures. Using standard operant techniques and 'go, no-go' successive discrimination paradigm, six BALB/c mice were trained to discriminate between presentation an air or odor stimulus (three for each odorants propanol propyl acetate). Two additional either vanillin propionic acid. Following criterion performance, mouse received 2000 trials overtraining. At completion overtraining one from acetate groups allocated as untreated. remaining inoculated 300 microl OBLV stock per nostril total 1.5 x 10(6) p.f.u. in 600 microl. 1 month rest, untreated animals again tested on their respective versus task. Untreated immediately performed at levels. In contrast, varied capacity odorant. Five showed massive disruption bulb, including death mitral cells; other was more modestly affected. addition, density innervation mucosa by substance P-containing trigeminal fibers is also affected inoculation. Those remained anosmic training had most severe reduction cell number P fiber among animals.