In vitro effects of nonsteroidal anti-inflammatory drugs on cyclooxygenase activity in dogs.

摘要: Objective—To establish an in vitro assay and determine the differential suppressive activity of non steroidal anti-inflammatory drugs (NSAID) on cyclooxygenase (COX)-1 COX-2 isoenzymes dogs. Procedure—COX was evaluated presence absence 4 NSAID (meloxicam, tolfenamic acid, carprofen, ketoprofen), using a canine monocyte/macrophage cell line that constitutively expresses COX-1, but can be induced to express when incubated with lipopolysaccharide. Inhibition prostaglandin E2 (PGE2) synthesis by each measured enzyme immunoassay attributed specific COX-1 or through assessment COX messenger RNA expression use northern blot analysis reverse transcription- polymerase chain reaction (RT-PCR). The selectivity drug from dose-response curves calculating ratio (COX- 1:COX-2) inhibitory concentration values basis concentrations reduced PGE2 50% model. Results—Meloxic...