Chromosomes 8, 12, and 17 copy number in Astler-Coller stage C colon cancer in relation to proliferative activity and DNA ploidy.
作者： Seth P. Harlow , Edoardo Colombo , Melissa G. Steiner , Kenneth D. Bauer
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摘要: Fluorescence in situ hybridization using centromere-specific DNA probes to chromosomes 8, 12, and 17 was applied 23 archival paraffin-embedded stage C colonic cancer specimens. Chromosome copy number related flow cytometric determinations of S-phase fraction ploidy. Three eight copies were observed at mean frequencies 28.7%, 37.8%, 20.9%, respectively. The frequency multiple chromosome 12 significantly greater than that for (P < 0.0025). single 8 0.0025 P 0.0005, respectively). Regarding the fourth quartile cases, defined on basis copies, proportion demonstrating moderate high proliferative activity greatly exceeded displaying low activity. same cases (most chromosomally aberrant) also generally demonstrated aneuploidy. results indicate a substantial degree karyotypic instability advanced colon cancer, particularly with
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