Escherichia coli Nissle 1917 facilitates tumor detection by positron emission tomography and optical imaging.

摘要: Purpose: Bacteria-based tumor-targeted therapy is a modality of growing interest in anticancer strategies. Imaging bacteria specifically targeting and replicating within tumors using radiotracer techniques optical imaging can provide confirmation successful colonization malignant tissue. Experimental Design: The uptake radiolabeled pyrimidine nucleoside analogues [ 18 F]FDG by Escherichia coli Nissle 1917 ( EcN ) was assessed both vitro vivo . to 4T1 breast monitored positron emission tomography (PET) imaging. accumulation the correlated with number bacteria. Optical based on bioluminescence done that encode luciferase genes under control an l-arabinose–inducible P BAD promoter system. Results: We showed be detected analogues, PET. Importantly, this paradigm does not require transformation bacterium reporter gene. provided lower contrast than F]FEAU due high FDG (nontreated) surrounding tissues. A linear correlation shown between viable F]FEAU, but F]FDG. presence also confirmed Conclusion: imaged PET, expression endogenous E. thymidine kinase, could translated patient studies for detection solid tumors. Bioluminescence provides low-cost alternative PET small animals.