Sulfite protects neurons from oxidative stress

摘要: Background and purpose Hydrogen sulfide (H2 S) polysulfides Sn ) are signalling molecules that mediate various physiological responses including cytoprotection. Their oxidized metabolite sulfite (SO3 2- is found in blood tissues. However, its role remains unclear. In this study, we investigated the cytoprotective effect of on neurons exposed to oxidative stress caused by high concentrations neurotransmitter glutamate, known as oxytosis. Experimental approach Concentrations well those cysteine GSH rats were measured HPLC. Cytoprotective effects primary cultures rat against oxytosis was examined WST-8 LDH cytotoxicity assays compared with H2 S, thiosulfate. Key results Free sulfite, present at approximately 2 μM brain, converts cystine more efficiently than S facilitates transport into cells. Physiological protected from accompanied increased intracellular probably due converting extracellular cysteine, . contrast, thiosulfate only slightly Conclusions implications Our data have shown be a novel molecule oxytosis, through maintaining levels milieu, leading GSH. Although there may adverse clinical sensitive individuals, our provide new insight therapeutic application neuronal diseases stress. Linked articles This article part themed section Chemical Biology Reactive Sulfur Species. To view other visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v176.4/issuetoc.