Catalysis of enzyme aggregates in organic solvents. An attempt at evaluation of intrinsic activity of proteases in ethanol

作者: Yasuhito Yamamoto , Hideo Kise

DOI: 10.1007/BF00138557

关键词:

摘要: Catalytic activities of α-chymotrypsin and subtilisin Carlsberg for transesterification N-acetyl-L-tyrosine methyl ester in ethanol markedly increased by decreasing the amount enzymes reaction mixtures. The results were kinetically attributed to changes KM kcat due formation smaller enzyme aggregates.

参考文章(17)
A Zaks, A M Klibanov, Enzymatic catalysis in nonaqueous solvents. Journal of Biological Chemistry. ,vol. 263, pp. 3194- 3201 ,(1988) , 10.1016/S0021-9258(18)69054-4
Robert S. Phillips, Michael S. Matthews, Eric Olson, Robert L. Von Tersch, Structural and stereochemical studies of esterification of aromatic amino acids by α-chymotrypsin in alcohol solvents Enzyme and Microbial Technology. ,vol. 12, pp. 731- 735 ,(1990) , 10.1016/0141-0229(90)90143-E
D. Cantacuzène, F. Pascal, C. Guerreiro, Synthesis of amino acid esters by papain Tetrahedron. ,vol. 43, pp. 1823- 1826 ,(1987) , 10.1016/S0040-4020(01)81493-9
Sanjay Kamat, Eric J. Beckman, Alan J. Russell, Role of diffusion in nonaqueous enzymology. 1. Theory. Enzyme and Microbial Technology. ,vol. 14, pp. 265- 271 ,(1992) , 10.1016/0141-0229(92)90149-I
J.L. Vidaluc, M. Baboulene, V. Speziale, A. Lattes, P. Monsan, Optimization of the enzymatic synthesis of amino acid esters. Reaction in polyphasic medium Tetrahedron. ,vol. 39, pp. 269- 274 ,(1983) , 10.1016/S0040-4020(01)91818-6
Evgeny N. Vulfson, Ghulam Ahmed, Iqbal Gill, Peter W. Goodenough, Igor A. Kozlov, Barry A. Law, L-Dopa ester synthesis catalyzed by α-chymotrypsin in organic solvents Biotechnology Letters. ,vol. 12, pp. 597- 602 ,(1990) , 10.1007/BF01030759
Robert G. Ingalls, Robert G. Squires, Larry G. Butler, Reversal of enzymatic hydrolysis: Rate and extent of ester synthesis as catalyzed by chymotrypsin and subtilisin Carlsberg at low water concentrations Biotechnology and Bioengineering. ,vol. 17, pp. 1627- 1637 ,(1975) , 10.1002/BIT.260171107
David E. Stevenson, Andrew C. Storer, Papain in organic solvents: Determination of conditions suitable for biocatalysis and the effect on substrate specificity and inhibition Biotechnology and Bioengineering. ,vol. 37, pp. 519- 527 ,(1991) , 10.1002/BIT.260370605