Sensitive electrochemical determination of miRNAs based on a sandwich assay onto magnetic microcarriers and hybridization chain reaction amplification.
作者： R.M. Torrente-Rodríguez , S. Campuzano , V. Ruiz-Valdepeñas Montiel , J.J. Montoya , J.M. Pingarrón
DOI: 10.1016/J.BIOS.2016.07.003
关键词:
摘要: A novel electrochemical approach for determination of miRNAs involving a sandwich hybridization assay onto streptavidin-magnetic beads (Strep-MBs), chain reaction (HCR) amplification and amperometric detection at disposable screen-printed carbon electrodes is reported. Using miRNA-21 as the target analyte, dynamic linear range from 0.2 to 5.0nM with 60pM (1.5fmol in 25μL) limit was obtained. The achieved sensitivity 24-fold higher than non-HCR conventional type MBs. Moreover, whole time lasted 1h 45min which remarkably shorter other reported methodologies. methodology exhibited full selectivity against non-complementary well an acceptable discrimination between homologous miRNA family members. applicability this demonstrated by determining mature total RNA (RNAt) extracted tumor cells human tissues.
sci-hub.se 参考文章(37)
Hadi Beitollahi, Abbas Gholami, Mohammad Reza Ganjali, Preparation, characterization and electrochemical application of Ag–ZnO nanoplates for voltammetric determination of glutathione and tryptophan using modified carbon paste electrode Materials Science and Engineering: C. ,vol. 57, pp. 107- 112 ,(2015) , 10.1016/J.MSEC.2015.07.034
Björn Koos, Gaëlle Cane, Karin Grannas, Liza Löf, Linda Arngården, Johan Heldin, Carl-Magnus Clausson, Axel Klaesson, M. Karoliina Hirvonen, Felipe M. S. de Oliveira, Vladimir O. Talibov, Nhan T. Pham, Manfred Auer, U. Helena Danielson, Johannes Haybaeck, Masood Kamali-Moghaddam, Ola Söderberg, Proximity-dependent initiation of hybridization chain reaction Nature Communications. ,vol. 6, pp. 7294- 7294 ,(2015) , 10.1038/NCOMMS8294
Qian Zhai, Yun He, Xiaolu Li, Jing Guo, Shengqiang Li, Gang Yi, A simple and ultrasensitive electrochemical biosensor for detection of microRNA based on hybridization chain reaction amplification Journal of Electroanalytical Chemistry. ,vol. 758, pp. 20- 25 ,(2015) , 10.1016/J.JELECHEM.2015.10.010
Tianxing Hu, Le Zhang, Wei Wen, Xiuhua Zhang, Shengfu Wang, Enzyme catalytic amplification of miRNA-155 detection with graphene quantum dot-based electrochemical biosensor Biosensors and Bioelectronics. ,vol. 77, pp. 451- 456 ,(2016) , 10.1016/J.BIOS.2015.09.068
Feng Li, Ting Hou, Wei Li, Xiaojuan Liu, Label-Free and Enzyme-Free Homogeneous Electrochemical Biosensing Strategy Based on Hybridization Chain Reaction: A Facile, Sensitive, and Highly Specific MicroRNA Assay. Analytical Chemistry. ,vol. 87, pp. 11368- 11374 ,(2015) , 10.1021/ACS.ANALCHEM.5B02790
Peng Miao, Yuguo Tang, Jian Yin, MicroRNA detection based on analyte triggered nanoparticle localization on a tetrahedral DNA modified electrode followed by hybridization chain reaction dual amplification Chemical Communications. ,vol. 51, pp. 15629- 15632 ,(2015) , 10.1039/C5CC05499K
Li Xu Yan, Qi Nian Wu, Yan Zhang, Yang Yang Li, Ding Zhun Liao, Jing Hui Hou, Jia Fu, Mu Sheng Zeng, Jing Ping Yun, Qiu Liang Wu, Yi Xin Zeng, Jian Yong Shao, Knockdown of miR-21 in human breast cancer cell lines inhibits proliferation, in vitro migration and in vivo tumor growth Breast Cancer Research. ,vol. 13, pp. 1- 14 ,(2011) , 10.1186/BCR2803
Shuyan Niu, Yu Jiang, Shusheng Zhang, Fluorescence detection for DNA using hybridization chain reaction with enzyme-amplification Chemical Communications. ,vol. 46, pp. 3089- 3091 ,(2010) , 10.1039/C000166J
Shahram Savad, Parvin Mehdipour, Mohammad Miryounesi, Reza Shirkoohi, Forouzandeh Fereidooni, Fatemeh Mansouri, Mohammad Hossein Modarressi, Expression analysis of MiR-21, MiR-205, and MiR-342 in breast cancer in Iran. Asian Pacific Journal of Cancer Prevention. ,vol. 13, pp. 873- 877 ,(2012) , 10.7314/APJCP.2012.13.3.873
Qing Yao, Hui Xu, Qian-Qian Zhang, Hui Zhou, Liang-Hu Qu, MicroRNA-21 promotes cell proliferation and down-regulates the expression of programmed cell death 4 (PDCD4) in HeLa cervical carcinoma cells. Biochemical and Biophysical Research Communications. ,vol. 388, pp. 539- 542 ,(2009) , 10.1016/J.BBRC.2009.08.044