Suppression of fibroblast proliferation by activated macrophages: Involvement of H2O2 and a non-prostaglandin E product of the cyclooxygenase pathway

摘要: Abstract Macrophages are considered promoters of fibroblast proliferation; however, suppression by activated macrophages may outweigh this effect. Activated murine peritoneal obtained in vivo exposure to C. parvum or vitro LPS-activation thioglycollate-induced macrophages, were tested for their effect on normal syngeneic dermal fibroblasts. -activated but not resident suppressed proliferation. Similarly, LPS, those unexposed Catalase partially protected fibroblasts from either macrophage population, suggesting involvement H 2 O the suppression. The cyclooxygenase inhibitors was also tested. Indomethacin, acetylsalicylic acid, eicosatetraynoic all macrophage-mediated Prostaglandins E , 1 and F 2α added exogenously at concentrations as high 10 −6 M failed suppress proliferation These findings suggest that a nonprostaglandin product pathway is involved