Menin, the multiple endocrine neoplasia type 1 gene product, exhibits GTP-hydrolyzing activity in the presence of the tumor metastasis suppressor nm23.
作者： Hiroko Yaguchi , Naganari Ohkura , Toshihiko Tsukada , Ken Yamaguchi
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摘要: MEN1, the gene responsible for multiple endocrine neoplasia type 1, is a tumor suppressor that encodes protein called menin, of unknown function with no homology to any known protein. Here we demonstrate menin interacts putative metastasis nm23H1/nucleoside diphosphate (NDP) kinase A in mammalian cells. Given roles nm23 as multi-functional protein, searched possible menin. Menin has effect on activities nm23; is, nucleoside kinase, or GTPase-activating Ras-related GTPase Rad. However, found hydrolyzes GTP GDP efficiently presence nm23, whereas alone shows little detectable activity. Furthermore, contains sequence motifs similar those all GTPases GTP-binding proteins and low affinity but specific binding GTP/GDP. These results suggest an atypical stimulated by nm23.
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