The inhibition by interleukin 1 of MSC chondrogenesis and the development of biomechanical properties in biomimetic 3D woven PCL scaffolds

摘要: Abstract Tissue-engineered constructs designed to treat large cartilage defects or osteoarthritic lesions may be exposed significant mechanical loading as well an inflammatory environment upon implantation in injured diseased joint. We hypothesized that a three-dimensionally (3D) woven poly(e-caprolactone) (PCL) scaffold seeded with bone marrow-derived mesenchymal stem cells (MSCs) would provide biomimetic properties early stages of in vitro culture the MSCs assembled functional, cartilaginous extracellular matrix (ECM). also these maintained even presence pro-inflammatory cytokine interleukin-1 (IL-1), which is found at high levels joints. MSC-seeded 3D scaffolds cultured chondrogenic conditions synthesized functional ECM rich collagen and proteoglycan content, reaching aggregate modulus ∼0.75 MPa within 14 days culture. However, pathophysiologically relevant IL-1 limited accumulation inhibited any increase over baseline values. On other hand, for 4 weeks prior exposure were protected from deleterious effects cytokine. These findings demonstrate significantly inhibits development maturation constructs; however, overall functionality engineered tissue can preserved through use recreate native articular cartilage.