Efficient Cre-loxP-induced mitotic recombination in mouse embryonic stem cells.

摘要: FLP/FRT-induced mitotic recombination provides a powerful method for creating genetic mosaics in Drosophila and discerning the function of recessive genes heterozygous individual. Here we show that can be reproducibly induced mouse embryonic stem (ES) cells, by Cre/loxP technology, at frequencies ranging from 4.2 x 10(-5) (Snrpn) to 7.0 10(-3) (D7Mit178) single allelic loxP sites, 5.0 10(-2) multiple lox after transient Cre expression. Notably, much occurs G2 is followed X segregation, where recombinant chromatids segregate away each other during mitosis. It segregation useful mosaic analysis because it produces clones homozygous mutant daughter cells mothers. Our studies confirm predictions made suggest will not limited organisms with strong pairing, forces (sister-chromatid cohesion) responsible are an elemental feature mitosis all eukaryotes. also mice feasible, least certain chromosomal regions.