Purification of a colony-stimulating factor from cultured pancreatic carcinoma cells.
作者： M Wu , J K Cini , A A Yunis
DOI: 10.1016/S0021-9258(18)50349-5
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摘要: Serum-free conditioned medium prepared from an established line of human pancreatic carcinoma (MIA PaCa-2) provides a rich source colony-stimulating factor (CSF). Two activities distinctly separable by isoelectrofocusing have been identified: high molecular weight CSF exhibiting greater activity in mouse bone marrow and low more active marrow. The has purified 1000-fold to apparent homogeneity two-step procedure including gel filtration chromatography. 50,000 isoelectric point 3.7 4.6. It is glycoprotein as shown periodic acid-Schiff stain exhibits than
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sci-hub.st 参考文章(10)
A W Burgess, J Camakaris, D Metcalf, Purification and properties of colony-stimulating factor from mouse lung-conditioned medium. Journal of Biological Chemistry. ,vol. 252, pp. 1998- 2003 ,(1977) , 10.1016/S0021-9258(18)71855-3
E R Stanley, P M Heard, Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells. Journal of Biological Chemistry. ,vol. 252, pp. 4305- 4312 ,(1977) , 10.1016/S0021-9258(17)40265-1
Donald Metcalf, Studies on colony formation in vitro by mouse bone marrow cells. I. Continuous cluster formation and relation of clusters to colonies. Journal of Cellular Physiology. ,vol. 74, pp. 323- 332 ,(1969) , 10.1002/JCP.1040740313
Purification of the protein that induces cell differentiation to macrophages and granulocytes FEBS Letters. ,vol. 37, pp. 149- 154 ,(1973) , 10.1016/0014-5793(73)80446-6
Ming-Chi Wu, Grace K. Arimura, Adel A. Yunis, Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry. ,vol. 16, pp. 1908- 1913 ,(1977) , 10.1021/BI00628A023
J. Kurland, R. Bockman, H. Broxmeyer, M. Moore, Limitation of excessive myelopoiesis by the intrinsic modulation of macrophage-derived prostaglandin E Science. ,vol. 199, pp. 552- 555 ,(1978) , 10.1126/SCIENCE.304600
Adel A. Yunis, Grace K. Arimura, David J. Russin, Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. International Journal of Cancer. ,vol. 19, pp. 128- 135 ,(1977) , 10.1002/IJC.2910190118
S. S. Fojo, M. C. Wu, M. A. Gross, Y. Purcell, A. A. Yunis, Purification and characterization of a colony stimulating factor from human lung. Biochemistry. ,vol. 17, pp. 3109- 3116 ,(1978) , 10.1021/BI00608A026
Jere P. Segrest, Richard L. Jackson, [5] Molecular weight determination of glycoproteins by polyacrylamide gel electrophoresis in sodium dodecyl sulfate Methods in Enzymology. ,vol. 28, pp. 54- 63 ,(1972) , 10.1016/0076-6879(72)28007-7
DH Ausprunk, J Das, Endotoxin-induced changes in human platelet membranes: morphologic evidence. Blood. ,vol. 51, pp. 487- 495 ,(1978) , 10.1182/BLOOD.V51.3.487.487