Purification and properties of colony-stimulating factor from mouse lung-conditioned medium.

摘要: Colony-stimulating factor, which specifically stimulates mouse bone marrow cells to proliferate in vitro and generate colonies of granulocytes, or macrophages, both, was purified 3500-fold from lung-conditioned medium. Analysis by discontinuous polyacrylamide gel electrophoresis the presence absence sodium dodecyl sulfate indicated that there a single protein component. All colony-stimulating activity coincident with band. The molecular weight factor estimated filtration approximately 29,000 23,000. specific medium bound concanavalin A-Sapharose, indicating it is glycoprotein. small percentage did not bind A-Sepharose appeared represent molecules lacked carbohydrate moieties required for binding this lectin. It necessary include low concentrations (less than 0.01%, v/v) polymers such as gelatin polyethylene glycol, nonionic detergents Triton X-100, all buffers used throughout purification scheme, otherwise lost solution. At high (greater 20 mug/ml) stimulated formation granulocytic, macrophage, mixed C57BL cells. As concentration decreased, frequency containing granulocytes also decreased. 70 pg/ml) only macrophage were stimulated.