Purification and characterization of a colony stimulating factor from human lung.
作者： S. S. Fojo , M. C. Wu , M. A. Gross , Y. Purcell , A. A. Yunis
DOI: 10.1021/BI00608A026
关键词:
摘要: Conditioned medium prepared from human autopsy lung tissue contains high level activity of colony stimulating factor which stimulates granulocytes and macrophage formation in both mouse bone marrow. The has been purified about 2250-fold by methods including hydroxylapatite chromatography, preparative gel electrophoresis, isoelectric focusing, filtration chromatography. final specific was 2.7 X 10(6) units/mg. a molecular weight 41 000 as determined filtration. It is stable at the pH range 6.5--10 56 degrees C for 30 min but sensitive to protease digestion periodate oxidation. On polyacrylamide it migrates alpha-globulin post-albumin region. Upon isoelectrofocusing appears heterogeneous with points 3.7--4.3. Treatment neuraminidase did not affect its activity, caused change electrophoretic mobility point. Antibody produced immunizing rabbits partially exerted strong inhibitory on well other sources serum, urine, placenta.
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