Intergeneric and intrageneric conjugal transfer of plasmid-encoded antibiotic resistance determinants in Leuconostoc spp.

摘要: Transfer of the broad-host-range resistance plasmids pIP501 and pAM beta 1 from Streptococcus faecalis to Leuconostoc dextranicum cremoris occurred between cells that were immobilized on nitrocellulose filters in presence DNase. spp. also when sanguis lactis used as donors. In addition, transfer was observed L. transconjugants S. faecalis. Expression erythromycin chloramphenicol determinants essentially equivalent donors transconjugants. Frequencies generally ranged 10(-4) 10(-7) per input donor cell. Intrageneric strains same approximate range. These data further extend host range demonstrate another example gene genus Leuconostoc.