Magnetic nanoparticles as gene delivery agents: enhanced transfection in the presence of oscillating magnet arrays.

摘要: Magnetic nanoparticle-based gene transfection has been shown to be effective in combination with both viral vectors and non-viral agents. In these systems, therapeutic or reporter genes are attached magnetic nanoparticles which then focused the target site/cells via high-field/high-gradient magnets. The technique efficient rapid for vitro compares well cationic lipid-based reagents, producing good overall levels lower doses shorter times. spite of its potential advantages (particularly vivo targeting), do not generally exceed those other order improve while maintaining inherent this technique, we have developed a novel, oscillating magnet array system adds lateral motion particle/gene complex promote transfection. Experimental results indicate that significantly enhances human airway epithelial cells compared static field techniques (p<0.005) lipids (p<0.001) tested. addition, it previously demonstrated magnetofection—rapid times requiring DNA than This method shows delivery vivo.