Long Non-Coding RNA LINC00491 Contributes to the Malignancy of Non-Small-Cell Lung Cancer via Competitively Binding to microRNA-324-5p and Thereby Increasing Specificity Protein 1 Expression.

摘要: Background A long non-coding RNA termed as intergenic non-protein coding 491 (LINC00491) has been validated an oncogene to promote cancer progression in colon adenocarcinoma. The goal of this study was determine the expression and carcinogenic functions LINC00491 non-small-cell lung (NSCLC). Besides, it aimed understand how affects malignant processes NSCLC cells. Methods investigated by bioinformatic analysis reverse transcription-quantitative PCR. After knockdown, cell counting kit-8 assay, flow cytometry, migration invasion detection assays well nude mice xenograft assay were conducted test roles Two online databases, StarBase 3.0 miRDB, utilized putative target miRNA LINC00491, prediction subsequently confirmed luciferase reporter immunoprecipitation PCR, Western blotting, rescue assays. Results overexpressed both tissues lines. Functional investigation revealed that depleted facilitated apoptosis decreased proliferation, migration, vitro. Additionally, downregulation impaired tumor growth vivo. Mechanistically, functioned a competing endogenous sponging microRNA-324-5p (miR-324-5p) miR-324-5p weakly expressed exerted tumor-suppressing actions during progression. Furthermore, specificity protein 1 (SP1) direct under regulation via miR-324-5p. Rescue experiments reconfirmed inhibition SP1 overexpression abrogated suppressive deficiency Conclusion promoted oncogenicity serving sponge, which further upregulated SP1. LINC00491/miR-324-5p/SP1 pathway disclosed new mechanism pathogenesis may provide effective targets for better treatment.