Correlation of live-cell imaging with volume scanning electron microscopy.

摘要: Live-cell imaging is one of the most widely applied methods in live science. Here we describe two setups for live-cell imaging, which can easily be combined with volume SEM correlative studies. The first procedure applies cell culture dishes a gridded glass support, used any light microscopy modality. second approach flow-chamber setup based on Ibidi μ-slides. Both strategies followed up serial blockface- or focused ion beam-scanning electron microscopy. Two types resin embedding after heavy metal staining and dehydration are presented making best use particular advantages each modality: classical en-bloc thin-layer plastification. latter only microscopy, but advantageous studying cell-interactions specific substrates, when substrate cannot removed. En-bloc has diverse applications both described scanning techniques. Finally, relocating interest discussed approaches respect to methods.