Generation of Transmission-Competent Human Malaria Parasites with Chromosomally-Integrated Fluorescent Reporters.

摘要: Malaria parasites have a complex life cycle that includes specialized stages for transmission between their mosquito and human hosts. These are an understudied part of the lifecycle yet targeting them is essential component effort to shrink malaria map. The parasite Plasmodium falciparum responsible majority deaths due malaria. Our goal was generate transgenic P. lines could complete produce fluorescent more in-depth high-throughput studies. Using zinc-finger nuclease technology engineer integration site, we generated three in which tdtomato or gfp were stably integrated into genome. Expression driven by either stage-specific peg4 csp promoters constitutive ef1a promoter. Phenotypic characterization these demonstrates they with high infection rates give rise stages. sufficiently bright intra-vital imaging, flow cytometry scalable screening chemical inhibitors inhibitory antibodies.