Target-specific outgrowth from human mesencephalic tissue grafted to cortex or ventricle of immunosuppressed rats.

摘要: Human fetal mesencephalic tissue was grafted to rats with unilateral lesions of the nigrostriatal pathway. The animals were immunosuppressed cyclosporine A. Grafts placed either into lateral ventricle ipsilateral lesion or in cingulate cortex above corpus callosum. grafts and newly formed fibers visualized by immunohistochemistry antibodies against tyrosine hydroxylase (TH) human Thy-1 glycoprotein. TH-positive covered total volume striatum when graft cortex. When transplant located ventricle, cells migrated from host striatum. No cell migration seen any other areas than Cortex septum sparsely reinnervated graft, but not a density higher that normally seen. Globus pallidus totally devoid fibers. cortex, fiber bundles penetrated through callosum striatum, arborize its dorsal parts, followed medial side ventral limbic areas, where network also formed. specific Thy-1-immunohistochemistry revealed positivity only on lesioned side. These data suggest dopamine neurons tissue, rat brain, can migrate specifically Furthermore, outgrowth occurred denervated host, avoiding are innervated nigral neurons, able reach distant target cells.