Differential effects of acellular embryonic matrices on pluripotent stem cell expansion and neural differentiation

摘要: Extracellular matrices (ECM) derived from pluripotent stem cells (PSCs) provide a unique tissue microenvironment that can direct cellular differentiation and regeneration, rejuvenate aged progenitor cells. The unlimited growth capacity of PSCs allows for the scalable generation PSC-secreted ECMs. Therefore, derivation characterization PSC-derived ECMs is critical importance in drug screening, disease modeling regeneration. In this study, 3-D were generated decellularized undifferentiated embryonic cell (ESC) aggregates (AGG), spontaneously differentiated embryoid bodies (EB), ESC-derived neural (NPC) aggregates. capacities different to proliferation reseeded mouse ESCs human induced (iPSCs) characterized. Proteomic analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed protein expression profiles reflected distinct niche properties each tested ECM group. iPSCs responded types with phenotypes. Cells grown on AGG-ECM displayed high levels markers Oct-4 Nanog, while NPC-ECM showed increased marker β-tubulin III. β-catenin EB-ECM, but reduced NPC-ECM, indicating possible role Wnt/β-catenin signaling cell-matrix interactions. This study demonstrates influence fate decisions providing spectrum microenvironments during development.