Photochrome Transmembrane Protein Bacteriorhodopsin from Purple Membranes of Halobacterium Halobium in Nano- and Biotechnologies

摘要: This paper presents improved method for isolation of photochrome transmembraine protein bacteriorhodopsin (output 5 mg from 100 g wet biomass) capable to transform light energy electrochemical generated protons H + and ??P. The was isolated purple membranes photo-organotrophic halobacterium Halobacterium halobium by cellular autolysis distilled water, processing bacterial biomass ultrasound at 22 KHz, alcohol extraction low high-weight molecular impurities, RNA, carotenoids lipids, solubilization with 0.5% (w/v) SDS-Na, fractionation MeOH column el permeation chromatography (GPC) the final on Sephadex G-200 0.1% SDS-Na 2.5 mM ETDA. homogeneity BR proved combination preparative analytical methods including elecrtophoresis in 12.5% PAAG regeneration apomembranes 13- trans -retinal. Keywords: , membranes, bacteriorhodopsin, biosynthesis, biomolecular electronics