PON1 paraoxonase activity is reduced during HDL oxidation and is an indicator of HDL antioxidant capacity
作者： Leila Jaouad , Christelle Milochevitch , Abdelouahed Khalil
DOI: 10.1080/1071576021000036614
关键词:
摘要: The aim of this study was to investigate the effect HDL oxidation on PON1 paraoxonase activity. Also, we were interested in investigating mechanism by which could be inactivated and correlation between its enzymatic activity antioxidant properties HDL. Three different systems used for oxidation: (1) induced THP1 cells, (2) copper ions at a concentration 10 microM, (3) *OH O2.- oxygen free radicals produced gamma-radiolysis. followed measurement lipid peroxide formation, determined measuring rate paraoxon hydrolysis. Our results show that is accompanied reduction extent inactivation depends both system used. rates significantly correlated (r = 0.93, p < 0.0054). oxidized loses protective toward LDL oxidation. action towards degradation 0.95, 0.04).
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