Identification and expression levels of pig miRNAs in skeletal muscle

摘要: Abstract MicroRNAs are a class of naturally occurring non-coding RNAs. Typically they ∼22 nucleotides long and suppress translation their targets genes. Several laboratories have attempted to identify miRNAs from pig muscle the bioinformatics strategies using ESTs proved be successful for this aim. In study we report an in silico identification ncRNA EST libraries focusing on novel further investigated differential expression pigs (known novel) by quantitative real-time PCR during pre- postnatal stage Commercial local breed Piau skeletal tissue. We identified two not yet described pigs: hsa-miR-1207-5p hsa-miR-665. Besides, found 288 target genes 214 hsa-miR-665; them, four specific Through analyses, differences were between stages genetics groups. The findings muscle-specific will helpful understanding function processing RNA future. differentially expressed breeds suggest that can used uncover phenotypic across different genetic