Preliminary Findings of DNA Transfer from Transgenic Plants to a Wild-Type Strain of Aspergillus niger

摘要: Nine different field isolates of bacteria and fungi were cocultivated with four species transgenic plants or leaf material expressing the hygromycin B resistance gene (hph) in sterile microcosms to determine possibility transfer events microorganisms. Significantly higher levels detected only for filamentous fungus Aspergillus niger after coculture, reisolation selection propagated material. The hph-gene, other vector sequences plant specific DNA some resistant colonies a recloned plasmid. However, mitotic stability seemed be low. Results transformation experiments transforming vectors gave rise assumption that high dose 35S-promoted hph-gene was necessary resist pressure. High cotransformation efficiencies recovering altered plasmids from uncut transformed clones indicated recombination activity fungal cells. possible uptake foreign might have occured during growth hyphae on A special role hyphal tips as location active protein secretion could assumed.