Non-radioactive multiple reverse transcription-PCR method used for low abundance mRNA quantification
作者： Mojca Benčina , Matic Legiša
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摘要: A non-radioactive multiple reverse transcription-PCR method for the relative quantitative analysis of low abundance mRNA is described. Chemiluminescent detection cDNA products amplified by PCR and incorporating biotin-labelled dCTP was used specific gene expression. After determining optimal concentrations RNA cDNA, tube-to-tube variations were minimised transcription amplification endogenous standard coding protease C from Aspergillus niger, together with transcript interest. All obtained data values comparing to internal pepC. By difference in level between two transformants different pkaC copy number cAMP-dependent protein kinase (PKA) catalytic subunit A. niger detected which accordance differences PKA enzymatic activity both strains.
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