Molecular typing and in vitro fluconazole susceptibility of Candida species isolated from diabetic and nondiabetic women with vulvovaginal candidiasis in India
作者： Ali Abdul Lattif , None
DOI: 10.1016/J.JMII.2011.01.020
关键词:
摘要: Background Candida albicans and glabrata are the major causes of vulvovaginal candidiasis (VVC) in Indian women with diabetes mellitus. Little information is available regarding genotyping species isolated from diabetic VVC. Methods In this study, a total 57 species, comprising , VVC, were genotyped tested for fluconazole susceptibility. Arbitrarily primed polymerase chain reaction (AP-PCR) was used to genotype C isolates, whereas Southern blot hybridization using repetitive element-2 (CARE-2) probe albicans. Results Genotyping showed that all isolates genetically heterogenous. The pattern DNA bands obtained after AP-PCR strains predominantly conformed A. vitro fluconazole-susceptibility testing Clinical Laboratory Standards Institute M27A2 protocol more than 93% susceptible. Conclusions Ninety-five percent analyzed different unrelated. analysis banding it resembled "A". found be susceptible fluconazole, minimum inhibitory concentrations ranging 0.5μg/mL 8μg/mL. This correlates use as first-choice antifungal treating VVC India.
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