Phospholipase and Aspartyl Proteinase Activities of Candida Species Causing Vulvovaginal Candidiasis in Patients with Type 2 Diabetes Mellitus.

摘要: Few research had investigated the secretion of phospholipase and aspartyl proteinase from Candida spp. causing infection in females with type 2 diabetes mellitus. This aimed to investigate prevalence vulvovaginal candidiasis (VVC) diabetic versus non-diabetic women compare ability identified isolates secrete phospholipases proteinases characterization their genetic profile. The study included 80 mellitus 100 within child-bearing period. strains were isolated by conventional microbiological methods API Candida. screened for extracellular activities culturing them on egg yolk bovine serum albumin media, respectively. Detection genes (SAP1 SAP8) (PLB1, PLB2) performed multiplex polymerase chain reaction. Our results indicated that vaginal was significantly higher among group nondiabetic (50% 20%, respectively) (p = 0.004). C. albicans most prevalent species followed glabrata both groups. No significant association between detected 0.262), whereas high exhibited found (82.5%) 0.000). Non-significant associations any tested or > 0.05). In conclusion, it is noticed incidence VVC increased. diabetics could be related increased production this patients.