Cutting Edge: DNA Polymerases μ and λ Are Dispensable for Ig Gene Hypermutation
作者： Barbara Bertocci , Annie De Smet , Eric Flatter , Auriel Dahan , Jean-Christophe Bories
DOI: 10.4049/JIMMUNOL.168.8.3702
关键词:
摘要: Mutations arising in Ig V genes during an immune response are most likely introduced by one or several error-prone DNA polymerases. Many of the recently described nonreplicative polymerases have intrinsic fidelity compatible with such activity, strongest candidates being polymerase (pol) eta, pol iota, zeta, and mu. We report this work that mice inactivated for either two related to beta (i.e., mu lambda) viable fertile display a normal hypermutation pattern.
jimmunol.org 参考文章(25)
Raul M Torres, Ralf Kühn, Laboratory Protocols for Conditional Gene Targeting ,(1997)
Myron F. Goodman, Brigette Tippin, The expanding polymerase universe. Nature Reviews Molecular Cell Biology. ,vol. 1, pp. 101- 109 ,(2000) , 10.1038/35040051
Xianmin Zeng, David B. Winter, Cynthia Kasmer, Kenneth H. Kraemer, Alan R. Lehmann, Patricia J. Gearhart, DNA polymerase η is an A-T mutator in somatic hypermutation of immunoglobulin variable genes Nature Immunology. ,vol. 2, pp. 537- 541 ,(2001) , 10.1038/88740
Marilyn Diaz, Laurent K. Verkoczy, Martin F. Flajnik, Norman R. Klinman, Decreased Frequency of Somatic Hypermutation and Impaired Affinity Maturation but Intact Germinal Center Formation in Mice Expressing Antisense RNA to DNA Polymerase ζ Journal of Immunology. ,vol. 167, pp. 327- 335 ,(2001) , 10.4049/JIMMUNOL.167.1.327
Jean-Claude Weil, Barbara Bertocci, Ahmad Faili, Said Aoufouchi, Stéphane Frey, Annie De Smet, Sébastien Storck, Auriel Dahan, Frédéric Delbos, Sandra Weller, Eric Flatter, Claude-Agnés Reynaud, Ig gene hypermutation: a mechanism is due. Advances in Immunology. ,vol. 80, pp. 183- 202 ,(2002) , 10.1016/S0065-2776(02)80015-3
T A Kunkel, The mutational specificity of DNA polymerase-beta during in vitro DNA synthesis. Production of frameshift, base substitution, and deletion mutations. Journal of Biological Chemistry. ,vol. 260, pp. 5787- 5796 ,(1985) , 10.1016/S0021-9258(18)89090-1
Peer Bork, Kay Hofmann, Philipp Bucher, Andrew F. Neuwald, Stephen F. Altschul, Eugene V. Koonin, A superfamily of conserved domains in DNA damage-responsive cell cycle checkpoint proteins. The FASEB Journal. ,vol. 11, pp. 68- 76 ,(1997) , 10.1096/FASEBJ.11.1.9034168
Kei-ichi Nagasawa, Kenzo Kitamura, Akihiro Yasui, Yuji Nimura, Kyoji Ikeda, Momoki Hirai, Akio Matsukage, Makoto Nakanishi, Identification and Characterization of Human DNA Polymerase β2, a DNA Polymerase β-Related Enzyme Journal of Biological Chemistry. ,vol. 275, pp. 31233- 31238 ,(2000) , 10.1074/JBC.M004263200
V. Poltoratsky, C. J. Woo, B. Tippin, A. Martin, M. F. Goodman, M. D. Scharff, Expression of error-prone polymerases in BL2 cells activated for Ig somatic hypermutation Proceedings of the National Academy of Sciences of the United States of America. ,vol. 98, pp. 7976- 7981 ,(2001) , 10.1073/PNAS.141222198
Patricia J. Gearhart, Richard D. Wood, Emerging links between hypermutation of antibody genes and DNA polymerases Nature Reviews Immunology. ,vol. 1, pp. 187- 192 ,(2001) , 10.1038/35105009