Nuclear Factor-κB Signature of Inflammatory Breast Cancer by cDNA Microarray Validated by Quantitative Real-time Reverse Transcription-PCR, Immunohistochemistry, and Nuclear Factor-κB DNA-Binding

摘要: Purpose: Inflammatory breast cancer (IBC) is the most aggressive form of locally advanced with high metastatic potential. In a previous study, we showed that IBC different compared non-IBC by cDNA microarray analysis. A list 756 genes significant expression differences between and was identified. In-depth functional analysis revealed presence number nuclear factor-κB (NF-κB) target elevated in versus non-IBC. This led to hypothesis NF-κB contributes phenotype IBC. The aim present study further investigate role Experimental Design: Immunohistochemistry DNA-binding experiments were done for all subunits (RelA, RelB, cRel, NFkB1, NFkB2) using specimens. Transcriptionally active dimers identified means coexpression addition, quantitative real-time reverse transcription-PCR eight genes, selected upon significant, 3-fold gene difference analysis, done. Results: We found overexpression transcription-PCR. statistically immunostained nuclei RelB ( P = 0.038) NFkB1 0.025) 0.031) higher ER− tumors ER+ tumors. Conclusions: transcription factor pathway probably possibly offers new options treatment patients diagnosed this cancer.