Immobilized hydrogels for screening of molecular interactions.

摘要: Spatially arrayed, high-density microarrays enable the rapid assessment of biological recognition events, and this information is widespread interest for those working in basic research laboratories as well clinic. Today, one can find DNA, protein, or small molecule arrays. Limitations with these systems include covalent modification target complement to array substrate, array- target-dependent setup conditions, multiple steps, loss hydration at surface. To overcome limitations, we have designed, prepared, evaluated immobilized hydrogels general screening chambers molecule-protein, protein-protein, nucleic acid-nucleic acid interactions. This biomaterial-based approach facile, rapid, requires only protocol, physically entraps within polymer network thus offers advantages over conventional chips.