Metabolic changes in follicular fluid of the dominant follicle in high-yielding dairy cows early post partum

摘要: Characteristics of the intrafollicular environment to which preovulatory oocyte is exposed may be one major factors determining subsequent fertility. The aim our study was examine what extent metabolic changes that occur in early post partum high-yielding dairy cows are reflected follicular fluid (FF) dominant follicle (>8 mm). Nine blood samples were taken per cow from nine between 7 days before and 46 after parturition. From Day 14 on together with sampling, FF largest collected same by means transvaginal aspiration. Serum analyzed using commercial clinical photometric chemistry assays for glucose, beta-hydroxybutyrate (beta-OHB), urea, total protein (TP), triglycerides (TG), non-esterified fatty acids (NEFA) cholesterol (TC). All lost body condition during experimental period (0.94+/-0.09 points) illustrating a negative energy balance period. In FF, glucose concentrations significantly higher TP, TG, NEFA TC lower than serum (P<0.05). beta-OHB, urea changed over time Throughout study, all metabolites similar FF. Especially beta-OHB correlations remarkably high. results present confirm typical adaptations can found shortly partum, and, therefore, affect quality both granulosa cells.