Aberrant Cell Growth Induced by Avian Winged Helix Proteins
作者： Jason S. Iacovoni , Markus P. Freyaldenhoven , Bettina S. Freyaldenhoven , Peter K. Vogt
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摘要: Abstract Winged helix transcription factors act as important regulators of embryonal development and tissue differentiation in vertebrates invertebrates. Identification the retroviral oncogene v-qin a member winged family showed that these developmental also have oncogenic potential. We used low-stringency hybridization chicken embryonic cDNA library to isolate clones coding for three (CWH) proteins, CWH-1, CWH-2, CWH-3. The CWH genes are transcribed tissue-restricted pattern adult tissues. proteins bind conserved DNA binding sites sequence-specific manner. Expression from replication-competent RCAS vectors induces changes morphology growth embryo fibroblasts. CWH-1 CWH-3 induce anchorage-independent agar. Chicken fibroblasts expressing constructs release viruses able elicit same cellular parental plasmid DNA. Our results suggest not only function but potential stimulate abnormal cell proliferation.
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