Search for varicella zoster virus DNA in saliva of healthy individuals aged 20-59 years.

摘要: All neurological and ocular complications of varicella zoster virus (VZV) reactivation can occur without rash. Virological verification requires detection VZV DNA or anti-VZV IgG antibody in cerebrospinal fluid (CSF), IgM serum CSF. If were readily detected other tissue patients with disease rash found to correlate tests listed above, more invasive such as lumbar puncture might be obviated. Saliva is a potential source DNA. To study the diagnostic value detecting saliva from disease, healthy adults searched for A single sample obtained by passive drool was centrifuged at 16,000 × g 20 mins. extracted supernatant cell pellet examined triplicate real time PCR. random 80 men women aged 20–59 years revealed no (Table 1), but uniformly positive (GAPdH) Because not individuals years-old, VZV-positive during neurologic may have significance. Further studies will determine whether correlates CSF disease. TABLE 1 Absence years Keywords: VZV, saliva, diagnosis, PCR INTRODUCTION Varicella ubiquitous neurotropic alphaherpesvirus. Primary infection usually produces (chickenpox), after which becomes latent ganglionic neurons along entire neuraxis [Gilden et al., 1983; Kennedy 1998; 1999]. Decades later, (shingles) further complicated meningoencephalitis, myelopathy, vasculopathy multiple disorders. Importantly, these 2010]. In instances well sine herpete (radicular pain absence rash), virological relies on less often, presence blood mononuclear cells samples (e.g., tears) shown standard obviated. Saliva [Gershon 2011]. First, present only all 54 acute herpes involving face, trunk, upper lower extremities [Mehta 2008], also peripheral facial palsy [Furuta 2000; 2001; 2005], indicative geniculate (Ramsay Hunt syndrome) herpete. up 28 days immunization people >60 history [Pierson Finally, frequency significantly higher than age-matched subjects [Nagel